Critical Velocity of Vortex Nucleation in Rotating Superfluid 3He-A

We have measured the critical velocity v_c at which 3He-A in a rotating cylinder becomes unstable against the formation of quantized vortex lines with continuous (singularity-free) core structure. We find that v_c is distributed between a maximum and minimum limit, which we ascribe to a dependence on the texture of the orbital angular momentum l(r) in the cylinder. Slow cool down through T_c in rotation yields l(r) textures for which the measured v_c's are in good agreement with the calculated instability of the expected l texture.Comment: 4 pages, 3 figure

Waterfalls as sources of small charged aerosol particles

In this study, we measured the mobility distributions of cluster and intermediate ions with an ion spectrometer near a waterfall. We observed that the concentration of negative 1.5–10 nm ions was one-hundred fold higher than a reference point 100 m away from the waterfall. Also, the concentration of positive intermediate ions was found to be higher than that at the reference point by a factor of ten. This difference was observed only at the smallest sizes; above 10 nm the difference was insignificant

The hydraulic jump as a white hole

In the geometry of the circular hydraulic jump, the velocity of the liquid in the interior region exceeds the speed of capillary-gravity waves (ripplons), whose spectrum is `relativistic' in the shallow water limit. The velocity flow is radial and outward, and thus the relativistic ripplons cannot propagating into the interior region. In terms of the effective 2+1 dimensional Painleve-Gullstrand metric appropriate for the propagating ripplons, the interior region imitates the white hole. The hydraulic jump represents the physical singularity at the white-hole horizon. The instability of the vacuum in the ergoregion inside the circular hydraulic jump and its observation in recent experiments on superfluid 4He by E. Rolley, C. Guthmann, M.S. Pettersen and C. Chevallier in physics/0508200 are discussed.Comment: 10 pages, no figures, references added, version submitted to JETP Letter

Vortex lines or sheets - what is formed in dynamic drives?

In isotropic macroscopic quantum systems vortex lines can be formed while in anisotropic systems also vortex sheets are possible. Based on measurements of superfluid 3He-A, we present the principles which select between these two competing forms of quantized vorticity: sheets displace lines if the frequency of the external field exceeds a critical limit. The resulting topologically stable state consists of multiple vortex sheets and has much faster dynamics than the state with vortex lines.Comment: RevTex, 5 pages, sumbitted to Phys. Rev. Let

Manipulation of Ag nanoparticles utilizing noncontact atomic force microscopy

We have developed a scheme to manipulate metallic aerosol particles on silicon dioxide substrates using an atomic force microscope. The method utilizes the noncontact mode both for locating and moving nanoparticles of size 10–100 nm. The main advantage of our technique is the possibility of “seeing” the moving particle in real time. Our method avoids well sticking problems that typically hamper the manipulation in the contact mode.Peer reviewe

The ribosomal P-stalk couples amino acid starvation to GCN2 activation in mammalian cells

The eukaryotic translation initiation factor 2α (eIF2α) kinase GCN2 is activated by amino acid starvation to elicit a rectifying physiological program known as the Integrated Stress Response (ISR). A role for uncharged tRNAs as activating ligands of yeast GCN2 is supported experimentally. However, mouse GCN2 activation has recently been observed in circumstances associated with ribosome stalling with no global increase in uncharged tRNAs. We report on a mammalian CHO cell-based CRISPR-Cas9 mutagenesis screen for genes that contribute to ISR activation by amino acid starvation. Disruption of genes encoding components of the ribosome P-stalk, uL10 and P1, selectively attenuated GCN2-mediated ISR activation by amino acid starvation or interference with tRNA charging without affecting the endoplasmic reticulum unfolded protein stress-induced ISR, mediated by the related eIF2α kinase PERK. Wildtype ribosomes isolated from CHO cells, but not those with P-stalk lesions, stimulated GCN2-dependent eIF2α phosphorylation in vitro. These observations support a model whereby lack of a cognate charged tRNA exposes a latent capacity of the ribosome P-stalk to activate GCN2 in cells and help explain the emerging link between ribosome stalling and ISR activation

Noise of a single electron transistor on a Si3N4 membrane

We have investigated the influence of electron-beam writing on the creation of charge trapping centers which cause 1/f noise in single electron transistors (SET). Two Al/AlOx/Al devices were compared: one where the SET is on a {100} silicon wafer covered by a 120-nm-thick layer of Si3N4, and another one in which the Si was etched away from below the nitride membrane before patterning the SET. The background charge noise was found to be 1×10 exp −3 e/√Hz at 10 Hz in both devices, independent of the substrate thickness.Peer reviewe

Predicting base editing outcomes using position-specific sequence determinants.

CRISPR/Cas base editors promise nucleotide-level control over DNA sequences, but the determinants of their activity remain incompletely understood. We measured base editing frequencies in two human cell lines for two cytosine and two adenine base editors at ∼14 000 target sequences and find that base editing activity is sequence-biased, with largest effects from nucleotides flanking the target base. Whether a base is edited depends strongly on the combination of its position in the target and the preceding base, acting to widen or narrow the effective editing window. The impact of features on editing rate depends on the position, with sequence bias efficacy mainly influencing bases away from the center of the window. We use these observations to train a machine learning model to predict editing activity per position, with accuracy ranging from 0.49 to 0.72 between editors, and with better generalization across datasets than existing tools. We demonstrate the usefulness of our model by predicting the efficacy of disease mutation correcting guides, and find that most of them suffer from more unwanted editing than pure outcomes. This work unravels the position-specificity of base editing biases and allows more efficient planning of editing campaigns in experimental and therapeutic contexts

Segregating YKU80 and TLC1 Alleles Underlying Natural Variation in Telomere Properties in Wild Yeast

In yeast, as in humans, telomere length varies among individuals and is controlled by multiple loci. In a quest to define the extent of variation in telomere length, we screened 112 wild-type Saccharomyces sensu stricto isolates. We found extensive telomere length variation in S. paradoxus isolates. This phenotype correlated with their geographic origin: European strains were observed to have extremely short telomeres (<150 bp), whereas American isolates had telomeres approximately three times as long (>400 bp). Insertions of a URA3 gene near telomeres allowed accurate analysis of individual telomere lengths and telomere position effect (TPE). Crossing the American and European strains resulted in F1 spores with a continuum of telomere lengths consistent with what would be predicted if many quantitative trait loci (QTLs) were involved in length maintenance. Variation in TPE is similarly quantitative but only weakly correlated with telomere length. Genotyping F1 segregants indicated several QTLs associated with telomere length and silencing variation. These QTLs include likely candidate genes but also map to regions where there are no known genes involved in telomeric properties. We detected transgressive segregation for both phenotypes. We validated by reciprocal hemizygosity that YKU80 and TLC1 are telomere-length QTLs in the two S. paradoxus subpopulations. Furthermore, we propose that sequence divergence within the Ku heterodimer generates negative epistasis within one of the allelic combinations (American-YKU70 and European-YKU80) resulting in very short telomeres